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Our Research Foci
Presynaptic Development and Memory Formation
What are the mechanisms underlying presynaptic development? To circumvent challenges studying identified neurons in mammals, our laboratory uses a Lymnaea Stagnalis model. We aim to uncover how voltage-dependent calcium channels and synaptic proteins are targeted during synapse formation and plasticity.
Targeting Calcium Release for Treatment of Hypoxic-ischemia
How can we develop more better treatments for hypoxic-ischemia? Our lab uses mouse models to investigate how targeting calcium channel function can support neuroprotection and recovery, aiming to develop effective pharmacological therapies.
Models
Lymnaea Stagnalis, mouse, cortical cell cultures
Methods
Electrophysiology, ratiometric calcium imaging, confocal imaging, recombinant DNA, gene expression analysis, microarrays, qRT-PCR, RT-PCR, immunohistochemistry, immunocytochemistry, Western blot, mass spectrometry, signal transduction characterization, proteomics, siRNA, stereotaxic brain surgery, behavioral tests.
Equipment
Patch-clamp rigs (Multiclamp 700, Axonpatch 200, digidata (1300 series)), motorized micromanipulators, calcium imaging (monochromator PTI, TILL), confocal microscope (Zeiss), culture hood, culture incubators, dissecting microscope, electrometer, fluorescence microscope, gel apparatus (BioRed), low- and high-speed centrifuge (-80°C), micropipette puller (Sutter), real-time/thermocycler, setups for electrophoresis (BioRed).
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